Single-Cell Genomics for Resolution of Conserved Bacterial Genes & Mobile Genetic Elements of the Human Intestinal Microbiota Using Flow Cytometry
Time: 11:00 am
day: Focus Day Track 2
Details:
- A method utilizing fluorescence-activated cell sorting for isolation of single bacterial cells, amplifying their genomes, screening them by 16S rRNA gene analysis, and selecting cells for genomic sequencing to provide nearly complete coverage of bacterial genomes when applied to isolates and partial genomes of bacterial species recovered from complex communities
- Utilizing this method to uncover conserved and variable genomic regions between samples from healthy and patient samples
- Show novel organisms discovered utilizing a flow cytometric approach to probing the microbiota
